Luminescence
Luminescence is one of the most sensitive analytical assay techniques available and also one of the most difficult to evaluate for assay and microplate reader suitability - but no longer. The MicroQC Plate excels with luminescence evaluation utilizing twelve microprocessor controlled to provide a stable and precise low-level light signal that can be used to evaluate a microplate reader's luminescence measurement system. Each of the twelve luminescence wells has a different lumen output providing an exceptionally stable calibration curve with which to evaluate a microplate reader.
The MicroQC Plate luminescence standards can be used to evaluate the precision and linearity of any microplate reader. Each of the luminescence wells has a known relative light intensity output and therefore these values can be used as standards for purposes of a linear regression linearity evaluation.
Unlike wet chemistry which can change from assay to assay, the MicroQC Plate luminescence standards are the same from minute to minute, or day to day. Therefore performance determinations can be made of single readers, or as shown to the right, comparisons of linearity and sensitivity can be made between readers. The MicroQC Plate has a dynamic range of more than four log with low values wells that approximate the lumen count from standard value glow luminescence assays.
As with the other analytical modes on the MicroQC Plate, the relative luminescence values of each well is stored on the MicroQC Plate's onboard memory and accessible through the ePaper menu screens.
