Assay Simulation
Luminescence assays are often flash type assays that produce a transient luminescence signal upon injection of a enzymatic substrate. One of the most popular flash luminescent assays is Promega's Dual-Luciferase* assay which produces a relatively steady high-intensity luminescent signal in the blue-green portion of the spectrum. The signal is subsequently quenched in the second part of the assay and the ratio between these values is the assay readout.
Flash luminescence assays are are great analytical tools but can be difficult to troubleshoot on a microplate reader. The MicroQC Plate can take the guess work out of flash assays with the assay simulation well. The MicroQC Plate simulates a typical flash assay using a microprocessor controlled LED to provide an increasing light signal over a span of ten seconds that then falls to zero and repeats. Using the MicroQC Plate simulation mode you can evaluate the kinetic capability of your microplate reader for reproducibility, dynamic range, and sensitivity.
Before you run your next kinetic luminescence assay you should use the MicroQC Plate's simulation mode to verify your microplate reader is up to the challenge before using expensive reagents or running an experiment only to suffer an equipment failure. Instead of guessing, you'll know that your reader is ready and capable of producing the data you need.
The luminescence simulation well is programmable via microprocessor control, meaning that it can simulate most any kinetic assay. New assay programming will be released to simulate some of the most popular assays on the market.
