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Microplate Reader Absorbance Assays

Absorbance is the most commonly used analytical method employed in microplate reader assays. Visible or ultraviolet, absorbance microplate assays are the mainstay of the life science laboratory.

The MicroQC Plate can help you determine if your microplate reader is properly reading absorbance assays and can be the cornerstone of your laboratory SOP and QC/QA procedures. With twelve absorbance wells ranging from 0.065 OD to over 2.50 OD, the MicroQC Plate completely covers the analytically relevant optical density range of absorbance assays. 

Phosentix uses NIST traceable standards. The MicroQC Plate absorbance  certificate data will be provided with NIST traceable values at 440nm, 465nm,492nm,546nm,590nm and 635nm. However the MicroQC Plate can be read throughout the visible spectrum at custom wavelengths if  required. Reference readings at 450nm and 650nm are also available on the certificate. by default.


By using the known absorbance values of the MicroQC Plate you can determine if your microplate reader meets precision, linearity, and accuracy metrics. The MicroQC Plate absorbance values will not change over time so you always can rely on it as a stable benchmark for QC procedures. The MicroQC Plate absorbance values at 440nm are stored on the device and can be accessed through the menus on the ePaper display. You'll never need worry about keeping up with certification paperwork and the values are automatically updated with yearly recertification of the MicroQC Plate.

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